منابع مشابه
p53 cuts off invading cells
In Focus • THE JOURNAL OF CELL BIOLOGY p53 cuts off invading cells T he tumor suppressor p53 does all it can to prevent oncogenes from inducing tumorigenesis, killing defective cells or pushing them into senes-cence. Sometimes, oncogenes manage to initiate tumor development in the presence of p53, but, even then, the tumor suppressor doesn't give up and focuses its efforts instead on limiting t...
متن کاملA Unique Egg Cortical Granule Localization Motif Is Required for Ovastacin Sequestration to Prevent Premature ZP2 Cleavage and Ensure Female Fertility in Mice
Monospermic fertilization is mediated by the extracellular zona pellucida composed of ZP1, ZP2 and ZP3. Sperm bind to the N-terminus of ZP2 which is cleaved after fertilization by ovastacin (encoded by Astl) exocytosed from egg cortical granules to prevent sperm binding. AstlNull mice lack the post-fertilization block to sperm binding and the ability to rescue this phenotype with AstlmCherry tr...
متن کاملLeukocytes drop anchor
B urkart et al. describe how a secreted protease helps egg cells avoid being fertilized by more than one sperm. Because polyspermy disrupts em-bryonic development, oocytes take several steps to ensure they only fuse with a single sperm. One key step is to prevent additional sperm from binding to the surface of an already-fertilized egg, a blockade that involves the release of secretory granules...
متن کاملOvastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy
The mouse zona pellucida is composed of three glycoproteins (ZP1, ZP2, and ZP3), of which ZP2 is proteolytically cleaved after gamete fusion to prevent polyspermy. This cleavage is associated with exocytosis of cortical granules that are peripherally located subcellular organelles unique to ovulated eggs. Based on the cleavage site of ZP2, ovastacin was selected as a candidate protease. Encoded...
متن کاملSperm - Egg Binding Protein or Proto - Oncogene ?
tized. The hybridization signal of the radiolabeled probe appears as white grains. All specimens were observed under dark field illumination after nuclear counterstain with hematoxylin. Immunostaining for factor Vill-related antigen (4) confirmed that injury was limited to endothelium. 23. Binding was carred out in buffer containing 50 mM NaCI, 5 mM MgCI2, 5% glycerol, 2.5 mM Hepes (pH 7.9), 1 ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2012
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.1971iti1